Eleven Queensland fruit flies have now been found in the Auckland suburb of Grey Lynn.
The latest find, which is an adult male, was found within a 200 metre radius of where the first fly was found last month.
Strict controls over movement of fresh fruit and vegetables within that area remain in place.
The Ministry for Primary Industries said all flies tested to date were genetically similar.
It said it was most likely that they were dealing with a localised population.