Holder Petra Kvitova suffered a shock 3-6 7-5 6-4 defeat by Serbia's former world number one Jelena Jankovic who produced an inspired display in the Wimbledon third round.
Second seed Kvitova breezed through her first two matches but after taking the first set against 30-year-old Jankovic, the Czech made too many errors allowing the 28th seed to triumph.
Jankovic has never been past the fourth round at the All England Club and admits that grass is not her favourite surface.
She admits she's played so poorly the last couple of years and playing on Centre Court against the defending champion was unbelievable.... the crowd was really great.
Twice champion Kvitova looked confident at the start, having dropped only three games in her first two matches, and when she broke Jankovic in the fourth game she looked set to progress.
The 25-year-old broke again in the third game of the second set on a sun-drenched Centre Court and went 4-2 ahead as Jankovic struggled to match her power around the court.
But the experienced Serb had battled through two three-set matches already at the tournament and knew how to dig deep as she fought back to 4-4 before forcing a decider.
The final set went with serve until Jankovic, who had begun to find the shots that saw her reach the U.S. Open final and top the rankings in 2008, broke Kvitova to close out the match.
In the last 16 Jankovic faces Poland's 13th seed Agnieszka Radwanska who beat Casey Dellacqua of Australia in straight sets.