Jarryd Hayne has been ruled out of Australia's upcoming Four Nations rugby league campaign with the Parramatta star to undergo intensive treatment on a groin injury.
Hayne was one of four players - along with departing Penrith skipper Petero Civoniceva, Gold Coast's Ashley Harrison and Titans-bound centre Jamal Idris - to be withdrawn from the Kangaroos train-on squad on Monday.
Eels five-eighth/fullback Hayne was expected to be included in the final squad, where his utility value would no doubt have come in handy for Test coach Tim Sheens.
He is not expected to undergo surgery, with Eels officials hopeful of having him fully fit ahead of pre-season training.
Civoniceva tore a pectoral muscle in his Panthers finale while Idris - who played in Australia's mid-year Test win over New Zealand - was unavailable due to wrist surgery ahead of his move from Canterbury to the Gold Coast.
Titans backrower Harrison's season ended prematurely when he hyper-extended his elbow against Brisbane in round 19, but ensuing shoulder surgery prior to the final round of the NRL regular season put paid to his green and gold bid.
Yesterday eight players were added to the train-on squad from which the Four Nations touring party to the United Kingdom and a Prime Minister's XIII to take on Papua New Guinea in Lae will be chosen.
As expected there were call-ups for likely Four Nations participants Kurt Gidley and Newcastle's Akuila Uate as well as North Queensland duo Matthew Scott and Johnathan Thurston, with those two sides having bowed out of the NRL finals over the weekend.
Also added to the squad were Knights forwards Chris Houston and Neville Costigan plus Cowboys duo Matt Bowen and Willie Tonga.
The squad will gather in Sydney on Thursday.