Three country residents are among 35 new cases diagnosed since Friday.
There are now 173 Victorians with confirmed swine flu, an increase of 74 in less than 24 hours.
The country cases are all north of Melbourne, with one in each of the shires of Macedon, Mitchell and Indigo.
"They are the first cases in rural and regional Victoria since human swine flu has arrived in our state," Health Minister Daniel Andrews said.
He would not say exactly where the cases were.
The spread outside Melbourne comes as authorities confirmed an Austin Hospital employee had contracted the virus, and doctors warned they were rapidly running out of equipment to protect themselves from infection.
Australian Medical Association (AMA) Victorian president Dr Harry Hemley said an urgent call had gone out for access to national stockpiles of masks, gowns, gloves and Tamiflu.
Mr Andrews said the Victorian government was liaising with the commonwealth to ensure that GPs had the resources needed to contain the virus.
More than 3,000 people are in quarantine in Victoria and seven schools remain closed.
Mr Andrews said 26 of the 35 new cases were children aged five to 18, but none has required hospital treatment.
