Bus operator GO Wellington says it will not run any services in the capital on Thursday.
The company has issued a notice of lock out to workers, which takes effect at midnight on Wednesday.
GO Wellington, the Tramways Union and the Manufacturing and Construction Workers Union are locked in an increasingly bitter pay dispute.
About 200 bus drivers walked off the job between 7.30am and 8.30am on Wednesday, following a breakdown in pay talks with Go Wellington.
In response to a lockout notice issued by the bus company, the union has announced a rolling strike unless the company presents a better pay offer.
The Manufacturing and Construction Workers Union has joined the action, with some of its members are not re-fuelling buses on Wednesday afternoon.
General secretary Graeme Clarke says people who have a problem with the strike action should talk to the Government.
Claim details
On Tuesday, the Tramways Union rejected an offer of a pay increase of 7% and demand between 8% to 12% instead.
It claims that pay for its 311 members was, in effect, cut last year when the company limited working hours.
The bus company says health and safety concerns were largely behind the cut in hours.
Chief executive Bruce Emson told Morning Report the situation follows three months of negotiations, and that their best pay offer is on the table.
Mr Emson said that includes a 7% pay increase for this year, a 4.3% increase for next year, as well as a one-off payment of $250 to each driver.
