A spokesperson for Colonel Muammar Gadaffi says the ousted leader is still in Libya, and is not part of a convoy of at least 50 vehicles that has crossed into neighbouring Niger.
The convoy is belived to be carrying gold and cash and is said to be headed for the capital, Niamey.
Niger officials also say Colonel Gaddafi is is not in the convoy, but say his security chief is among several former Libyan officials who have arrived in Niamey.
They say the man, Mansour Daw, entered the country on Sunday and travelled via the desert city of Agadez, the BBC reports.
Meanwhile, opposition forces in Libya have concluded their talks with tribal elders in Bani Walid aimed at the peaceful surrender of the town.
Bani Walid is one of four towns and cities still controlled by Gaddafi supporters.
The senior negotiator for the elders says he is confident a peaceful end to the stand-off is in sight.